Vectorology - GEG Tech top picks

Here the authors describe a simple protocol for the production of lentiviral vectors (LV) by triple transfection into HEK-293 cells, and the subsequent purification of virions from the cellular media. The current protocol is versatile, and can be easily modified to fit the specific needs of the researcher in order to produce relatively high-titer LV vectors which can be used to transduce a wide variety of cells both in vitro and in vivo.

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