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Vectorology - GEG Tech top picks
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BigField GEG Tech's insight:
The authors created a number of custom designed AAV and lentiviral vectors that were optimized for large transgenes, by minimizing DNA sequences that were not essential, utilizing short promoter sequences and utilizing a very short 3′ untranslated sequence. 
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In this works, the authors design a doxycycline-inducible self-inactivating (SIN) lentiviral vectors for endothelial-specific expression. They tested two different design and containing two transgenes either the same or opposite orientation with respect to the vector sequence. The results showed that the vector with these two expression cassettes placed in opposite directions was optimal, characterized by a robust induction of the transgene expression (17.7- to 73-fold) in the presence of doxycycline in several endothelial cell lines, but without leakiness when uninduced.
www.geg-tech.com/Vectors