Vectorology - GEG Tech top picks

 Thanks to shRNA lentiviral vectors which target promyelocytic leukemia (PML), the authors show PML depletion induces telomere damage, nuclear and chromosomal abnormalities, and senescence. This work provides a novel view of the physiologic function of PML, which participates in telomeres surveillance in normal cells. 

www.geg-tech.com/Vectors

The authors used a LentiCRISPR-sgTRF2-1 to knockdown TERF2. They observe an  exacerbation of mitotic telomere deprotection which increases the ratio of cells that died during mitotic arrest and sensitized cancer cells to mitotic poisons. They propose a crisis pathway wherein chromosome fusions induce mitotic arrest, resulting in mitotic telomere deprotection and cell death, thereby eliminating precancerous cells from the population.

www.geg-tech.com/Vectors