In this study, the authors used an lentiviral vector to generate a human pancreatic cancer cell line (AsPC-1) which stably overexpresses the human serine protease inhibitor Kazal type 1 (SPINK1). They demonstrated that a stable AsPC-1 cell line capable of overexpressing SPINK1 had been successfully created, and that the proliferative capacity of AsPC-1 pancreatic cancer cells was significantly raised by SPINK1 upregulation as well as the ability of a single AsPC-1 cell to grow into a colony.
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In this study, the authors used an lentiviral vector to generate a human pancreatic cancer cell line (AsPC-1) which stably overexpresses the human serine protease inhibitor Kazal type 1 (SPINK1). They demonstrated that a stable AsPC-1 cell line capable of overexpressing SPINK1 had been successfully created, and that the proliferative capacity of AsPC-1 pancreatic cancer cells was significantly raised by SPINK1 upregulation as well as the ability of a single AsPC-1 cell to grow into a colony.
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