Vectorology - GEG Tech top picks

The authors report the feasibility of a novel reprogramming process based on vectors derived from the non-integrating vaccine strain of measles virus (MV). The reprogramming vector cocktail with the OCT4-expressing MV vector and SOX2-, KLF4- and cMYC-expressing lentiviral vectors efficiently transduced human skin fibroblasts and formed iPSC colonies.

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Lentiviral Vectors (LV) pseudotyped with the measles virus (MV) is a powerfull gene transfer tool with many applications. However the problem with MV pseudotyped LV vectors is the difficulty to obtain vector productions with high titer. In this study, the scientists have adapted an anion-exchange membrane chromatography method to concentrate MV glycoprotein-pseudotyped LV vectors. In this manner, up to 60% of the input vectors with an up to 5300-fold reduction in volume was achieved.

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