Vectorology - GEG Tech top picks

In this work, the authors used lentiviral vectors to investigate non-coding sequences adjacent to the human GLUCAGON and ARX genes, which are expressed in islet α cells. Elements with high evolutionary conservation were cloned into lentiviral vectors to direct fluorescent reporter expression in primary human islets.They found that rat Glucagon promoter was not specific for human α cells, but that addition of human GLUCAGON untranslated region (UTR) sequences substantially enhanced specificity of labeling in both cultured and transplanted islets to a degree not previously reported, to our knowledge. Specific transgene expression from these cis-regulatory sequences in human α-cells should enable targeted genetic modification and lineage tracing.

www.geg-tech.com/Vectors