Lentiviral Vectors (LV) pseudotyped with the measles virus (MV) is a powerfull gene transfer tool with many applications. However the problem with MV pseudotyped LV vectors is the difficulty to obtain vector productions with high titer. In this study, the scientists have adapted an anion-exchange membrane chromatography method to concentrate MV glycoprotein-pseudotyped LV vectors. In this manner, up to 60% of the input vectors with an up to 5300-fold reduction in volume was achieved.
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Lentiviral Vectors (LV) pseudotyped with the measles virus (MV) is a powerfull gene transfer tool with many applications. However the problem with MV pseudotyped LV vectors is the difficulty to obtain vector productions with high titer. In this study, the scientists have adapted an anion-exchange membrane chromatography method to concentrate MV glycoprotein-pseudotyped LV vectors. In this manner, up to 60% of the input vectors with an up to 5300-fold reduction in volume was achieved.
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