BACKGROUND:Lentiviral vectors have been successfully used for human skin cell gene transfer studies. Defining the selection of integration sites for retroviral vectors in the host genome is crucial in risk assessment analysis of gene therapy
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Vectorology - GEG Tech top picks
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BigField GEG Tech's insight:
The scientists constructed a lentiviral vector which expressed EGFP under the control of Figal promoter. Figla is specifically expressed in female mice oocyte, in this way, this vector could allow to study the development and the differentiation of germ cells derived from stem cells. 
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In the present study, we undertook an experimental approach which enabled direct correlation of the cell cycle stage of the target cell with the integration profile of LVs. The results indicate that approximately 10% of integration events occurred in actively transcribed genes and that the cell cycle stage of target cells affects integration pattern.
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In this study, the barrier activity of an insulator element from the human ankyrin-1 gene was analyzed in a lentiviral vector carrying an antisickling human β-globin gene. The presence of the Ankyrin insulator element did not change transgene expression in human hematopoietic cells in short-term erythroid culture or in vivo in primary murine transplants. However, analysis in secondary recipients showed that the lentiviral vector with the Ankyrin element preserved transgene expression, whereas expression from the vector lacking the Ankyrin insulator decreased in secondary recipients.
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BigField GEG Tech's insight:
The authors have constructed several lentiviral vector to express the factor VIII under the control of different promoters : MND, EF1 and glycoprotein-1bα (G1B). Their results show that only the G1B-FVIII vector achieves long-term and efficient expression, resulting in partial correction of hemophilia A. This work illustrates the importance of the choice of the promoter in lentiviral vector system. For this reason, GEG-tech offers sets of vectors ready to used which allow to select the best promoter in a given experimental context. www.geg-tech.com/Vectors/index.php/sets.html?element_tryout=10
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In this study, 874 unique lentiviral vector integration sites in human HaCaT keratinocytes after long-term culture were identified and analyzed with the online tool GTSG-QuickMap and SPSS software.
The data indicated that lentiviral vectors showed integration site preferences for genes and gene-rich regions.
This study will likely assist in determining the relative risks of the lentiviral vector system and in the design of a safe lentiviral vector system in the gene therapy of skin diseases.