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Vectorology - GEG Tech top picks
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BigField GEG Tech's insight:
The authors designed a lentivral vector to deliver CRISPR/Cas9 system in endothelial cells to disrupt the gene for the class II transactivator (CIITA). They showed the observed loss-of-function arises from bi-allelic gene disruption in CIITA that leaves other essential properties of the cells intact, including self-assembly into blood vessels in vivo, and that the altered phenotype can be rescued by re-introduction of CIITA expression. 
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The scientists used lentiviral vectors to deliver CRISPR/Cas9 to disrupt the gene for the class II transactivator (CIITA). They observed that the loss-of-function arises from bi-allelic gene disruption in CIITA that leaves other essential properties of the cells intact, including self-assembly into blood vessels in vivo, and that the altered phenotype can be rescued by re-introduction of CIITA expression.
www.geg-tech.com/Vectors